2G1L

Crystal structure of the FHA domain of human kinesin family member C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-02-07
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	107.620, 107.620, 82.995
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.602
R-factor	0.216
Rwork	0.215
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wln
RMSD bond length	0.015
RMSD bond angle	1.365
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.690
High resolution limit [Å]	2.600	5.590	2.600
Rmerge	0.121	0.039	0.940
Number of reflections	9139	1024	877
<I/σ(I)>	8.3
Completeness [%]	99.8
Redundancy	20.9	19	21.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1.4M malonate, pH 7, vapor diffusion, sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP