2G0T

Crystal structure of a putative nucleotide binding protein (tm0796) from Thermotoga maritima at 2.67 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979224, 0.979508, 0.885567
Spacegroup name	P 41 3 2
Unit cell lengths	191.634, 191.634, 191.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.570 - 2.670
R-factor	0.208
Rwork	0.207
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	2.367
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.928	29.930	2.740
High resolution limit [Å]	2.670	11.940	2.670
Rmerge	0.188	0.059	0.892
Total number of observations		3805	14093
Number of reflections	34565	442	2426
<I/σ(I)>	3.7	10.8	0.8
Completeness [%]	99.6	93.7	96.5
Redundancy	9.5	8.6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	6.6	277	0.2M NH4Formate, 20.0% PEG-3350, No Buffer, pH 6.6, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K