2G0F

Crystal Structure of P144A mutant of E.coli CcmG protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	CONVENTIONAL Cu
Temperature [K]	293
Detector technology	CCD
Collection date	2005-12-24
Detector	BRUKER APEX
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	35.416, 47.783, 85.432
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.720 - 2.200
R-factor	0.186
Rwork	0.186
R-free	0.23800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2b1k
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	SMART
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.104	0.594
Number of reflections	7932
Completeness [%]	99.8	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	2.0M ammonium sulfate, 0.1M HEPES buffer (pH=8.0),2%(w/v) PEG 4K, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K