2G0E
Structure of QacR Multidrug Transcriptional Regulator Bound to Trivalent and Bivalent Diamidine Drugs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-06 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 170.470, 170.470, 94.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 2.880 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.29900 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.080 | 0.308 |
| Number of reflections | 33410 | |
| <I/σ(I)> | 12.3 | 3.8 |
| Completeness [%] | 17.3 | 96.4 |
| Redundancy | 5.7 | 5.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 2.8M ammonium sulfate, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
