2G0D
Nisin cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Collection date | 2005-06-23 |
| Wavelength(s) | 0.9497 |
| Spacegroup name | I 41 |
| Unit cell lengths | 154.852, 154.852, 50.798 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.620 - 2.210 |
| R-factor | 0.18957 |
| Rwork | 0.186 |
| R-free | 0.23301 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.320 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.620 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.344 |
| Number of reflections | 30883 | |
| <I/σ(I)> | 36 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 3.5 M Sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
