2FZL
Structure of C-terminal domain of Archaeoglobus fulgidus XPB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.115869 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 172.859, 172.859, 172.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.2481 |
Rwork | 0.249 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fwr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.068 | 0.629 |
Number of reflections | 10024 | 978 |
<I/σ(I)> | 55.18 | 5.06 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25.5 | 25.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.8 | 303 | Ammonium sulphate, isopropanol, CAPS, pH 9.8, VAPOR DIFFUSION, HANGING DROP, temperature 303K |