2FZ2

Structure of Turnip Yellow Mosaic Virus at 100 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-12-11
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 64 2 2
Unit cell lengths	511.530, 511.530, 303.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.120 - 2.900
R-factor	0.277
Rwork	0.276
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1auy
RMSD bond length	0.013
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.120	2.650
High resolution limit [Å]	2.610	2.610
Number of reflections	225845
<I/σ(I)>	7.49	-0.41
Completeness [%]	35.3	0.5
Redundancy	3.2	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	298	1.0-1.7 M NH4PO4, 0.1 M MES buffer, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.4	298	1.0-1.7 M NH4PO4, 0.1 M MES buffer, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.4	298	1.0-1.7 M NH4PO4, 0.1 M MES buffer, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K