2FYX
Crystal structure of a putative transposase (dr_0177) from deinococcus radiodurans r1 at 1.90 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-25 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 |
Unit cell lengths | 66.787, 66.787, 81.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.240 - 1.900 |
R-factor | 0.16159 |
Rwork | 0.160 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f4f chain A |
RMSD bond length | 0.017 |
RMSD bond angle | 1.703 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.915 | 28.920 | 1.950 |
High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
Rmerge | 0.045 | 0.029 | 0.547 |
Total number of observations | 1585 | 6798 | |
Number of reflections | 31895 | 358 | 2360 |
<I/σ(I)> | 9.9 | 19.7 | 1.3 |
Completeness [%] | 100.0 | ||
Redundancy | 3.9 | 4.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6.5 | 277 | 2.0M (NH4)2SO4, 0.2M NaCl, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |