2FYV
Golgi alpha-mannosidase II complex with an amino-salacinol carboxylate analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-24 |
Detector | BRUKER |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.806, 108.635, 137.384 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.600 - 1.900 |
R-factor | 0.162 |
Rwork | 0.162 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hty |
RMSD bond length | 0.020 |
RMSD bond angle | 1.900 |
Data reduction software | PROTEUM PLUS |
Data scaling software | SAINT |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.092 | 0.409 |
Number of reflections | 81152 | |
<I/σ(I)> | 12.35 | 2.68 |
Completeness [%] | 99.6 | 95.4 |
Redundancy | 5.81 | 3.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Tris, PEG 6K, MPD, NaCl, pH 7, vapor diffusion, hanging drop, temperature 298K |