2FYV
Golgi alpha-mannosidase II complex with an amino-salacinol carboxylate analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-24 |
| Detector | BRUKER |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.806, 108.635, 137.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 1.900 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.900 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | 0.409 |
| Number of reflections | 81152 | |
| <I/σ(I)> | 12.35 | 2.68 |
| Completeness [%] | 99.6 | 95.4 |
| Redundancy | 5.81 | 3.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Tris, PEG 6K, MPD, NaCl, pH 7, vapor diffusion, hanging drop, temperature 298K |
