2FYG
Crystal structure of NSP10 from Sars coronavirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2005-09-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 3 |
Unit cell lengths | 108.874, 108.874, 108.874 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.460 - 1.800 |
R-factor | 0.19764 |
Rwork | 0.196 |
R-free | 0.23295 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.488 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.460 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.600 |
Number of reflections | 19037 | |
<I/σ(I)> | 2.36 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.7 | 3.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 2.0 M AMMONIUM FORMATE, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |