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2FYG

Crystal structure of NSP10 from Sars coronavirus

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.2.1
Synchrotron siteALS
Beamline8.2.1
Temperature [K]200
Detector technologyCCD
Collection date2005-09-29
DetectorADSC QUANTUM 210
Wavelength(s)1.0
Spacegroup nameI 21 3
Unit cell lengths108.874, 108.874, 108.874
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution44.460 - 1.800
R-factor0.19764
Rwork0.196
R-free0.23295
Structure solution methodSAD
RMSD bond length0.014
RMSD bond angle1.488
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]44.4601.840
High resolution limit [Å]1.8001.800
Rmerge0.0480.600
Number of reflections19037
<I/σ(I)>2.36
Completeness [%]100.0100
Redundancy5.73.14
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.52772.0 M AMMONIUM FORMATE, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

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