2FY6
Structure of the N-terminal domain of Neisseria meningitidis PilB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.81 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.870, 45.169, 59.094 |
| Unit cell angles | 90.00, 111.87, 90.00 |
Refinement procedure
| Resolution | 18.620 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.182 |
| R-free | 0.21900 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Auto-Rickshaw |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | 0.405 |
| Number of reflections | 11090 | 1088 |
| <I/σ(I)> | 6.9 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 3.5M Ammonium Sulfate, Tris-HCl 50mM pH 8.0, 6mg/mL protein, pH 7.5, microbatch, temperature 293K |
