2FXV

Bacillus subtilis Xanthine Phosphoribosyltransferase in Complex with Guanosine 5'-monophosphate (GMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-19
Detector	MARRESEARCH
Wavelength(s)	0.813
Spacegroup name	P 21 21 21
Unit cell lengths	53.570, 54.280, 138.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.850 - 2.050
Rwork	0.211
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l1r
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	22917
<I/σ(I)>	16.7	3
Completeness [%]	89.3	91.4
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 8000, Calcium Chloride, Sodium Acetate, Morpholine ethanesulfonic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K