2FXT
Crystal Structure of Yeast Tim44
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-10-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9789, 0.9793, 0.9743 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 124.250, 124.250, 77.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.550 - 3.200 |
| R-factor | 0.316 |
| Rwork | 0.316 |
| R-free | 0.36800 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.550 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 5555 | |
| Completeness [%] | 89.1 | 89.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 4.1M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
