2FXL
Urate oxidase from aspergillus flavus complexed with allantoin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.962 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.364, 96.086, 105.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.760 |
| R-factor | 0.1906 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r4s |
| RMSD bond length | 0.036 |
| RMSD bond angle | 0.079 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.900 | 1.850 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.070 | |
| Number of reflections | 33267 | |
| <I/σ(I)> | 18.7 | 5.1 |
| Completeness [%] | 98.1 | 97.6 |
| Redundancy | 4.8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | CRYSTALLIZATION CONDITIONS: 8.5MG/ML PROTEIN, EXCESS URIC ACID, 5-7% W/V PEG 8000, 100MM TRIS/HCL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
