2FXK

Crystal structure of the macro-domain of human core histone variant macroH2A1.1 (form A)

Replaces:  1ZQ0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-17
Detector	MARRESEARCH
Wavelength(s)	0.931
Spacegroup name	P 21 21 2
Unit cell lengths	90.770, 98.270, 42.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.910 - 2.540
R-factor	0.21
Rwork	0.207
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zr5
RMSD bond length	0.014
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.910	2.700
High resolution limit [Å]	2.540	2.540
Number of reflections	12857
<I/σ(I)>	16.12	4.48
Completeness [%]	99.0	96.3
Redundancy	6.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	10% PEG8000, 0.1M NACL, 0.1M TRIS/ HCL PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K, pH 7.50