2FX6
bovine trypsin complexed with 2-aminobenzamidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 280 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.710, 63.200, 69.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 1.570 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.21500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.720 |
| Data reduction software | CrystalClear ((d*trek)) |
| Data scaling software | CrystalClear (D*TREK (MSC/RIGAKU)) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.650 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.049 | 0.270 |
| Number of reflections | 39770 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.4 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | benzamidine, magnesium sulfate, CaCl2, 0.1 M Tris-HCl, pH 8.1-8.2, VAPOR DIFFUSION, temperature 298K |
