2FX5
Pseudomonas mendocina lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1991-01-01 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.500, 58.500, 145.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 3.100 |
| Phasing software | PROTEIN |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.080 |
| Number of reflections | 19100 |
| Completeness [%] | 95.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 50 mM sodium acetate, 18% saturated potassium tartrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
