2FWM
Crystal Structure of E. coli EntA, a 2,3-dihydrodihydroxy benzoate dehydrogenase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2005-08-02 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.585, 66.643, 109.732 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
Rwork | 0.186 |
R-free | 0.21400 |
Structure solution method | MAD |
RMSD bond length | 1569.000 |
RMSD bond angle | 2129.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.059 | 0.298 |
Number of reflections | 14485 | |
Completeness [%] | 95.4 | 70 |
Redundancy | 4.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 277 | 2% PEG4000, 2% ethylene glycol, 300 mM AmSO4, 50 mM NaSuccinate, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.00 |