2FWE
crystal structure of the C-terminal domain of the electron transfer catalyst DsbD (oxidized form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 98.2 |
| Detector technology | CCD |
| Collection date | 2003-08-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.75141 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.265, 45.961, 73.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| Rwork | 0.165 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | modified version of 2TRX (see publication) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 13029 | |
| <I/σ(I)> | 19 | 3.3 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277.15 | 0.3M sodium acetate, 0.1M sodium iodide, 40% PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
