2FW5
Diheme cytochrome c from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.025, 45.193, 34.357 |
| Unit cell angles | 90.00, 105.87, 90.00 |
Refinement procedure
| Resolution | 24.000 - 2.000 |
| R-factor | 0.17275 |
| Rwork | 0.171 |
| R-free | 0.21024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fwt |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.939 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.169 |
| Number of reflections | 9175 | |
| <I/σ(I)> | 10.8 | 4.05 |
| Completeness [%] | 97.6 | 87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25-30% PEG 8000, 0.1M MnSO4, 0.1M sodium cacodylate buffer pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
