2FW0
Apo Open Form of Glucose/Galactose Binding Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-14 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.800, 70.300, 112.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 1.550 |
R-factor | 0.165 |
Rwork | 0.165 |
R-free | 0.17800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2FVY |
RMSD bond length | 0.025 |
RMSD bond angle | 2.200 |
Data reduction software | SAINT |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.039 | 0.180 |
Number of reflections | 65759 | |
<I/σ(I)> | 8.31 | |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50mM NaCitrate, 2.5M AmSO4, 2.0 M Malonate cryoprotectant, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |