2FVY
High Resolution Glucose Bound Crystal Structure of GGBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.83 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.989, 36.238, 80.101 |
| Unit cell angles | 90.00, 124.50, 90.00 |
Refinement procedure
| Resolution | 9.000 - 0.920 |
| R-factor | 0.1179 |
| Rwork | 0.110 |
| R-free | 0.12960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Salmonella Typhimuruim GGBP |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.032 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.000 | 0.950 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Rmerge | 0.045 | 0.247 |
| Number of reflections | 187424 | |
| <I/σ(I)> | 36.8 | 2.7 |
| Completeness [%] | 95.3 | 74.7 |
| Redundancy | 4.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 25% PEG 4000, 5% Glycerol, 0.1M HEPES PH 7.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
