2FVG

Crystal structure of Endoglucanase (tm1049) from THERMOTOGA MARITIMA at 2.01 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-31
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979815
Spacegroup name	P 21 21 21
Unit cell lengths	63.150, 67.540, 82.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.500 - 2.010
R-factor	0.20478
Rwork	0.203
R-free	0.24423
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.539
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.500	29.500	2.080
High resolution limit [Å]	2.010	4.320	2.010
Rmerge	0.085	0.057	0.398
Number of reflections	22815	4610	2077
<I/σ(I)>	11.06	28.35	1.97
Completeness [%]	85.9
Redundancy	3.49

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	10.5	277	0.2M NaCl, 20.0% PEG-8000, 0.1M CAPS pH 10.5 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	10.5	277	0.2M NaCl, 20.0% PEG-8000, 0.1M CAPS pH 10.5 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K