2FV4
NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain
Spectrometer
Experimental method: SOLUTION NMR
Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
1 | Bruker | AVANCE | 600 |
Experiment
experiment id | conditions id | solution id | Experiment type |
1 | 1 | 1 | TROSY versions of HNCA, HNCACB and HNCO; 3D 15N-TOCSY-HSQC. |
2 | 1 | 2 | TROSY versions of HNCA, HNCACB and HNCO; 3D 15N-TOCSY-HSQC. |
3 | 1 | 3 | 3D 15N-NOESY, 3D 13C-NOESY, and 3D 15N-edited 13C-filtered NOESY; 2D spin echo difference experiments for measuring three-bond J(C'C) and J(NC); standard HNCO-based 3D experiments for measuring one-bond J(NH) and J(C'CA). |
4 | 1 | 4 | 3D 15N-NOESY, 3D 13C-NOESY, and 3D 15N-edited 13C-filtered NOESY; 2D spin echo difference experiments for measuring three-bond J(C'C), J(NC) and J(CC); standard HNCO-based 3D experiments for measuring one-bond J(NH). |
5 | 1 | 5 | Standard HNCO-based 3D experiments for measuring residual one-bond D(NH) and D(C'CA). |
6 | 1 | 6 | Standard HNCO-based 3D experiments for measuring residual one-bond D(NH). |
NMR Sample
conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
1 | 7 | ambient | 303 |
Conformers
Conformers Calculated Total Number | 30 |
Conformers Submitted Total Number | 20 |