2FV4

NMR solution structure of the yeast kinetochore Spc24/Spc25 globular domain

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE		600

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	TROSY versions of HNCA, HNCACB and HNCO; 3D 15N-TOCSY-HSQC.
2	1	2	TROSY versions of HNCA, HNCACB and HNCO; 3D 15N-TOCSY-HSQC.
3	1	3	3D 15N-NOESY, 3D 13C-NOESY, and 3D 15N-edited 13C-filtered NOESY; 2D spin echo difference experiments for measuring three-bond J(C'C) and J(NC); standard HNCO-based 3D experiments for measuring one-bond J(NH) and J(C'CA).
4	1	4	3D 15N-NOESY, 3D 13C-NOESY, and 3D 15N-edited 13C-filtered NOESY; 2D spin echo difference experiments for measuring three-bond J(C'C), J(NC) and J(CC); standard HNCO-based 3D experiments for measuring one-bond J(NH).
5	1	5	Standard HNCO-based 3D experiments for measuring residual one-bond D(NH) and D(C'CA).
6	1	6	Standard HNCO-based 3D experiments for measuring residual one-bond D(NH).

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	7	ambient	303

Conformers

Conformers Calculated Total Number	30
Conformers Submitted Total Number	20