2FV2

Crystal Structure Analysis of human Rcd-1 conserved region

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	200
Detector technology	CCD
Collection date	2005-03-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9568, 0.9795, 0.9797, 0.9407
Spacegroup name	P 1
Unit cell lengths	68.508, 68.963, 69.361
Unit cell angles	94.16, 117.26, 91.91

Refinement procedure

Resolution	27.260 - 2.200
R-factor	0.217
Rwork	0.217
R-free	0.27900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.036
Number of reflections	53307
Completeness [%]	95.8	89.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	298	100 mM MES pH5.7, 115 mM MnSO4, 17% PEG 3350, 1% glucose, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K