2FV2
Crystal Structure Analysis of human Rcd-1 conserved region
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2005-03-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9568, 0.9795, 0.9797, 0.9407 |
Spacegroup name | P 1 |
Unit cell lengths | 68.508, 68.963, 69.361 |
Unit cell angles | 94.16, 117.26, 91.91 |
Refinement procedure
Resolution | 27.260 - 2.200 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.27900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.036 | |
Number of reflections | 53307 | |
Completeness [%] | 95.8 | 89.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 100 mM MES pH5.7, 115 mM MnSO4, 17% PEG 3350, 1% glucose, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |