2FUV
Phosphoglucomutase from Salmonella typhimurium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-05 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.658, 104.864, 125.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.330 - 2.000 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.20010 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.433 |
| Data scaling software | SCALEPACK |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.330 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.101 | 0.374 |
| Number of reflections | 60537 | |
| <I/σ(I)> | 21.7 | 2.98 |
| Completeness [%] | 80.4 | 27.2 |
| Redundancy | 8.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2 M magnesium chloride, o.1 M Bis-Tris, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
