2FUP

Crystal structure of a putative flagella synthesis protein flgn (pa3352) from pseudomonas aeruginosa at 1.48 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9797, 0.9999, 0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	62.531, 62.531, 80.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 1.480
R-factor	0.19
Rwork	0.188
R-free	0.21800
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.713
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.740	29.740	1.560
High resolution limit [Å]	1.480	4.680	1.480
Rmerge	0.081	0.038	0.764
Number of reflections	27261
<I/σ(I)>	5.8	12.6	1
Completeness [%]	100.0	99.7	100
Redundancy	6.8	6.3	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	4	277	20.0% MPD, 0.1M Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K