2FUK
Crystal structure of XC6422 from Xanthomonas campestris: a member of a/b serine hydrolase without lid at 1.6 resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98977 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.980, 79.490, 38.120 |
| Unit cell angles | 90.00, 108.00, 90.00 |
Refinement procedure
| Resolution | 24.800 - 1.600 |
| R-factor | 0.22 |
| Rwork | 0.210 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.800 | |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 27545 | |
| Completeness [%] | 99.1 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.1M Na cacodylate pH6.5, 1.6M (NH4)2SO4, 2% PEG 400, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
