2FU8
Zinc-beta-lactamase L1 from stenotrophomonas maltophilia (d-captopril complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 105.011, 105.011, 196.615 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.200 - 1.800 |
| R-factor | 0.16093 |
| Rwork | 0.160 |
| R-free | 0.18252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sml |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.386 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.240 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.114 | 0.416 |
| Number of reflections | 56045 | |
| <I/σ(I)> | 5.3 | 1.8 |
| Completeness [%] | 90.9 | 90.9 |
| Redundancy | 2.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280 | AS, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
