2FU7
Zinc-beta-lactamase L1 from stenotrophomonas maltophilia (Cu-substituted form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979667 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 105.350, 105.350, 196.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.370 - 1.850 |
R-factor | 0.16474 |
Rwork | 0.163 |
R-free | 0.19535 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sml |
RMSD bond length | 0.015 |
RMSD bond angle | 1.457 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.420 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.076 | 0.445 |
Number of reflections | 55212 | |
<I/σ(I)> | 9.4 | 1.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280 | AS, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 280K |