2FU4
Crystal Structure of the DNA binding domain of E.coli FUR (Ferric Uptake Regulator)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979637 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 38.500, 159.270, 28.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.420 - 1.800 |
| R-factor | 0.17406 |
| Rwork | 0.172 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.276 |
| Data reduction software | XNEMO |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 17195 | |
| <I/σ(I)> | 17.72 | 6.2 |
| Completeness [%] | 99.4 | 97.1 |
| Redundancy | 8.5 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100mM sodium acetate, 30%(v/v) PEG 200 containing 100mM CdCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
