2FU3

Crystal structure of gephyrin E-domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-24
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	108.820, 156.195, 51.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.202
R-free	0.27300
Structure solution method	and MAD
RMSD bond length	0.021
RMSD bond angle	1.541
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.095	0.058	0.572
Number of reflections	35018	1350	1173
<I/σ(I)>	16.4
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	311	0.1M Tris-HCl (pH 7.5-8.5), 0.1M Na Acetate, and 25-30% PEG 4000, vapor diffusion, hanging drop, temperature 311K, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	7.5	311	0.1M Tris-HCl (pH 7.5-8.5), 0.1M Na Acetate, and 25-30% PEG 4000, vapor diffusion, hanging drop, temperature 311K, VAPOR DIFFUSION, HANGING DROP