2FU2
Crystal structure of protein SPy2152 from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.087, 30.280, 35.855 |
| Unit cell angles | 90.00, 113.26, 90.00 |
Refinement procedure
| Resolution | 34.940 - 2.150 |
| R-factor | 0.16111 |
| Rwork | 0.157 |
| R-free | 0.25389 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.813 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.087 | 0.283 |
| Number of reflections | 4682 | |
| <I/σ(I)> | 30.26 | 3.62 |
| Completeness [%] | 91.1 | 58.6 |
| Redundancy | 6.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 290 | 20 % PEG 8000, Mes pH 6.0, Ca(OAc)2 , VAPOR DIFFUSION, SITTING DROP, temperature 290K |
