2FTZ

Crystal structure of Geranyltranstransferase (EC 2.5.1.10) (tm0161) from THERMOTOGA MARITIMA at 1.90 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9801
Spacegroup name	P 43 21 2
Unit cell lengths	138.594, 138.594, 46.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 1.900
R-factor	0.17
Rwork	0.169
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rtr
RMSD bond length	0.017
RMSD bond angle	1.430
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.620	29.620	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.062	0.022	0.475
Number of reflections	36302
<I/σ(I)>	7.4	25.9	1.6
Completeness [%]	100.0		100
Redundancy	6.9	5.9	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	10.5	277	0.2M NaCl, 20.0% PEG-8000, 0.1M CAPS pH 10.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K