2FTR
Crystal structure of an ethyl tert-butyl ether d (ethd) family protein (bh0200) from bacillus halodurans c-125 at 1.40 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-11 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.530, 45.454, 51.423 |
Unit cell angles | 90.00, 99.93, 90.00 |
Refinement procedure
Resolution | 17.610 - 1.400 |
R-factor | 0.14921 |
Rwork | 0.148 |
R-free | 0.17400 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.329 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 17.610 | 17.610 | 1.440 |
High resolution limit [Å] | 1.400 | 6.260 | 1.400 |
Rmerge | 0.069 | 0.063 | 0.246 |
Number of reflections | 36413 | ||
<I/σ(I)> | 6.3 | 10.2 | 3.1 |
Completeness [%] | 83.9 | ||
Redundancy | 3.4 | 3.7 | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6 | 277 | 10.0% Glycerol, 5.0% PEG-1000, 30.0% PEG-600, 0.1M MES pH 6.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |