2FTK
berylloflouride Spo0F complex with Spo0B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-07-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.467, 118.331, 168.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.860 - 3.050 |
| Rwork | 0.232 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f51 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.009 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.860 | 3.240 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 28073 | |
| Completeness [%] | 98.6 | 90.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.1 | 297 | 0.5 M KCl, 25% PEG2000, 100mM Tris HCl ph8.1, VAPOR DIFFUSION, temperature 297K |
