2FT8
Structure of Cu(I)azurin, pH8, with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM"
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 51.953, 51.953, 75.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.800 - 1.550 |
| R-factor | 0.142 |
| Rwork | 0.139 |
| R-free | 0.19100 |
| Structure solution method | Direct refinement from refined AZAMI-F Cu(II) structure |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.609 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.064 | 0.358 |
| Total number of observations | 17358 | |
| Number of reflections | 15561 | 2162 |
| <I/σ(I)> | 7.3 | 2.2 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 8.3 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1MM MES, 20% PEG6000, 0.2M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
