2FT1
Bacteriophage HK97 Head II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.689 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 1009.804, 1009.804, 732.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.900 |
| Rwork | 0.297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ohg |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.970 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.203 | 0.439 |
| Number of reflections | 1737016 | 52838 |
| <I/σ(I)> | 2.6 | 1.1 |
| Completeness [%] | 52.1 | 31.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 100mM Sodium Acetate pH 4.6, 300mM Magnesium Acetate Hexahydrate, 25% 1,6-Hexanediol, pH 5.9, vapor diffusion, hanging drop, temperature 293K |
