2FSW
Crystal Structure of the Putative Transcriptional Regualator, MarR family from Porphyromonas gingivalis W83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.971, 82.241, 116.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.780 - 2.160 |
| R-factor | 0.2078 |
| Rwork | 0.203 |
| R-free | 0.25004 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.289 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.780 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.071 | 0.254 |
| Number of reflections | 18091 | |
| <I/σ(I)> | 13.7 | 4.76 |
| Completeness [%] | 92.9 | 69.3 |
| Redundancy | 10.9 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES pH 7.5, 1.4M sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
