2FSR
Crystal Structure of the Acetyltransferase from Agrobacterium tumefaciens str. C58
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97912 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.468, 106.778, 42.099 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.880 - 1.520 |
R-factor | 0.18147 |
Rwork | 0.179 |
R-free | 0.19935 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.880 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.081 | 0.320 |
Number of reflections | 30447 | |
<I/σ(I)> | 12.8 | 4.97 |
Completeness [%] | 96.8 | 81.4 |
Redundancy | 12.9 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Bis-tris, 0.2M NH4(OAC), 25% PEG3350, 4% Jeffamine M-600, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |