2FSQ
Crystal Structure of the Conserved Protein of Unknown Function ATU0111 from Agrobacterium tumefaciens str. C58
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-20 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 33.926, 35.603, 51.189 |
Unit cell angles | 89.70, 79.12, 69.44 |
Refinement procedure
Resolution | 21.880 - 1.400 |
R-factor | 0.15843 |
Rwork | 0.154 |
R-free | 0.20032 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.293 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.880 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.099 | 0.235 |
Number of reflections | 39939 | |
<I/σ(I)> | 18 | 6.5 |
Completeness [%] | 92.6 | 75.6 |
Redundancy | 4.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M Bis-tris, 0.2M Na(OAC), 30% PEG8K, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K |