2FSN
Crystal structure of Ta0583, an archaeal actin homolog, complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.703, 92.840, 73.659 |
| Unit cell angles | 90.00, 89.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| Rwork | 0.283 |
| R-free | 0.35900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.869 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.659 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.045 | 0.414 |
| Total number of observations | 8454 | |
| Number of reflections | 15505 | 2241 |
| <I/σ(I)> | 11 | 1.4 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM Bis-Tris pH 6.5, 15-17% PEG-2000MME, 14-16% ethylene glycol, 10mM ADP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
