2FSK
Crystal structure of Ta0583, an archaeal actin homolog, SeMet data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.482, 104.321, 62.546 |
| Unit cell angles | 90.00, 96.15, 90.00 |
Refinement procedure
| Resolution | 62.020 - 2.100 |
| Rwork | 0.174 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.594 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE (2.01) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.321 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.082 | 0.338 |
| Total number of observations | 21583 | |
| Number of reflections | 39827 | 5787 |
| <I/σ(I)> | 6.4 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM Bis-Tris pH 6.5, 9-17% PEG-2000MME, 21-22% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
