2FSJ
Crystal structure of Ta0583, an archaeal actin homolog, native data
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.95436 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.265, 50.762, 123.391 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.695 - 1.900 |
Rwork | 0.196 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.695 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.078 | 0.292 |
Total number of observations | 9580 | |
Number of reflections | 26096 | 3666 |
<I/σ(I)> | 6.4 | 2.2 |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 3.7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM Bis-Tris pH 6.5, 15-17% PEG-2000MME, 21-22% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |