2FSG
Complex SecA:ATP from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97889 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.382, 89.478, 163.349 |
| Unit cell angles | 90.00, 100.73, 90.00 |
Refinement procedure
| Resolution | 19.610 - 2.200 |
| Rwork | 0.208 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 59.740 | 2.320 |
| High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
| Rmerge | 0.077 | 0.020 | 0.644 |
| Total number of observations | 13042 | 49535 | |
| Number of reflections | 106818 | 3539 | 14458 |
| <I/σ(I)> | 8.5 | 29.7 | 1.1 |
| Completeness [%] | 96.9 | 99.5 | 92 |
| Redundancy | 3.5 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
