2FSF

Escherichia coli SecA, the preprotein translocase dimeric ATPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 1 21 1
Unit cell lengths	75.030, 90.171, 163.049
Unit cell angles	90.00, 100.48, 90.00

Refinement procedure

Resolution	19.980 - 2.000
Rwork	0.213
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.029
RMSD bond angle	2.210
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		2.100
High resolution limit [Å]	2.000	6.000	2.000
Rmerge	0.042	0.034	0.352
Number of reflections	140049	4162	16916
<I/σ(I)>	12.49	32.1	2.3
Completeness [%]	97.1	97	86.7
Redundancy	3.2		2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1
1
1