2FS7

Crystal Structure of Apo-Cellular Retinoic Acid Binding Protein Type II At 1.55 Angstroms Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	77
Detector technology	CCD
Collection date	2005-04-01
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	34.475, 37.220, 57.597
Unit cell angles	73.09, 75.74, 87.22

Refinement procedure

Resolution	50.000 - 1.550
R-factor	0.149
Rwork	0.144
R-free	0.20100
Structure solution method	Rigid Body Refinement
RMSD bond length	0.019
RMSD bond angle	1.742
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.042	0.157
Number of reflections	35956	2650
<I/σ(I)>	23.7	3.36
Completeness [%]	92.3	68.1
Redundancy	2.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	30% (w/v) PEG 8000, 0.1 M Tris-HCl pH 8.0, and 0.2 M Sodium Acetate, vapor diffusion, hanging drop, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	30% (w/v) PEG 8000, 0.1 M Tris-HCl pH 8.0, and 0.2 M Sodium Acetate, vapor diffusion, hanging drop, temperature 298K