2FS6
Crystal Structure of Apo-Cellular Retinoic Acid Binding Protein Type II At 1.35 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.449, 37.056, 56.527 |
| Unit cell angles | 72.68, 76.37, 87.28 |
Refinement procedure
| Resolution | 17.240 - 1.350 |
| R-factor | 0.154 |
| Rwork | 0.148 |
| R-free | 0.20400 |
| Structure solution method | Rigid body refinement |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.775 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.042 | 0.261 |
| Number of reflections | 50380 | 3219 |
| <I/σ(I)> | 14.56 | 1.77 |
| Completeness [%] | 88.4 | 56.5 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% (w/v) PEG 8000 (4000), 0.1 M TRIS-HCl pH 8.0 (8.5), and 0.2 M Sodium Acetate, vapor diffusion, hanging drop, temperature 298K |
