2FS3

Bacteriophage HK97 K169Y Head I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 43 21 2
Unit cell lengths	1009.643, 1009.643, 729.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 4.200
R-factor	0.384
Rwork	0.384
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ohg
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	RAVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.270
High resolution limit [Å]	4.200	4.200
Rmerge	0.162	0.396
Number of reflections	1679512	28999
<I/σ(I)>	4.1	1.5
Completeness [%]	63.0	21.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	100mM Sodium Cacodylate, 300mM Magnesium Acetate Hexahydrate, 25% 1,6-Hexanediol, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	100mM Sodium Cacodylate, 300mM Magnesium Acetate Hexahydrate, 25% 1,6-Hexanediol, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K