2FRP
Bacteriophage HK97 Expansion Intermediate IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.18076 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 1008.967, 1008.967, 728.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 7.500 |
| R-factor | 0.421 |
| Rwork | 0.421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ohg |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RAVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 7.630 |
| High resolution limit [Å] | 7.500 | 7.500 |
| Rmerge | 0.201 | 0.258 |
| Number of reflections | 306300 | 4813 |
| Completeness [%] | 65.0 | 20.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 293 | 50mM Citric Acid pH 4.0, 0.2M Magnesium Acetate Hexahydrate, 0.1M KCl, 25% MPD, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
